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TargeTronics, LLC


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LtrB TargeTron Vectors
EcI5 TargeTron Vectors

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LtrB Intron Design
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TargeTron Design

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TargeTron Description

TargeTron is a simple genetic tool for targeted bacterial gene knockout(s)
Computer algorithm for group II intron RNA design

TargeTron News

September 10, 2012

Peter Enyeart, a graduate student from Dr. Ellington lab engineered TargeTrons for efficiently delivering lox sites to defined genomic loci without using selectable markers. He was able to demonstrate large-scale genomic modifications, including insertions, deletions, inversions, and cut-and-paste operations.

Enyeart, P.J., Chirieleison, S.M., Dao, M.N., Perutka, J., Keatinge-Clay, A.T. & Ellington A.D. Generalized bacterial genome editing using mobile group II introns and Cre-lox. (in submission)

September 1, 2012

Dr. Roland Saldanha, UES Program Manager & Research Scientist, developed select-agent-compliant TargeTron vectors for use in Francisella tularensis Shu4 that permit rapid systematic disruption of pathogen genes and direct evaluation of the role of these genes in an infection model using primary human macrophages to identify potential therapeutic and vaccine candidates.

Saldanha, R.J., Prarat, M.V., Pemberton, A., Busbee, T., Perutka, J., Bohning, J., Kawamoto, S., Taylor, D., Adiga, U., Lambowitz, A.M., Ellington, A.D., Su, S., Hassett, D.J. & Lamkin, T.J. Facile Targeted Gene Disruption in the Select Agent Francisella tularensis Schu S4 and the Live Vaccine Strain (LVS). (in submission)