TARGETRONICS, LLC
is dedicated to TargeTron (group II intron RNA) design, support, and innovation
TargeTron Description
TargeTron is a simple genetic tool for targeted bacterial gene knockout(s)
- TargeTron inserts site-specifically into double-stranded DNA
- TargeTron insertion site is recognized mainly by base-pairing of the intron RNA
- The best insertion sites in the DNA sequence are predicted computationally on this website
- TargeTron itegration mechanism is highly efficient and specific
- TargeTron has permanent and conditional gene disruption capabilities
- TargeTron enables overnight gene disruption without selection
- Minimal screening to isolate mutants is required
- TargeTron supports Cre-mediated genome editing
Computer algorithm for group II intron RNA design
- Identifies multiple rank-ordered TargeTron insertion sites in the DNA sequence
- Verifies the specificity of TargeTron insertion sites in the reference genome
- Designs primers and gBlocks to modify TargeTrons for insertion into these sites
- Allows you to define a restriction enzyme sequence in your primers and gBlocks