LtrB Intron Design Site The LtrB intron recognizes different target sequence than the EcI5 intron The EcI5 intron is more efficient than the LtrB intron Enter your email address : File to upload : (help) Enzyme sequence for the IBS primer/gBlock : HindIII XbaI XhoI (help) Reference genome : none Clostridium phytofermentans ISDg, complete genome E.coli str. K-12 substr. MG1655 NC_000913 F.tularensis SCHU S4 NC_006570 C. hiranonis DSM 13275 C.difficile 630 NC_009089 & NC_008226 C.acetobutylicum ATCC 824 NC_003030 & NC_001988 C.botulinum A str. ATCC 3502 NC_009495 & NC_009496 C.perfringens str. 13 NC_003366 & NC_003042 C.tetani E88 NC_004557 & NC_004565 Rickettsia rickettsii str. Sheila Smith NC_009882.1 Rickettsia parkeri NC_017044.1 Chlamydia trachomatis 434/Bu NC_010287.1 Chlamydophila pneumoniae NC_000922 Akkermansia muciniphila NZ_AP021898 Chlamydia trachomatis serovar D UW-3/Cx NC_000117 Chlamydia muridarum Nigg NC_002620 Listeria monocytogenes 10403S NC_017544.1 (help) Name of the target (14 characters max., letters and numbers only, no spaces, no underscore) : Authorization code : (case sensitive)