EcI5 Intron Design Site The EcI5 intron recognizes different target sequence than the LtrB intron The EcI5 intron is more efficient than the LtrB intron Enter your email address : File to upload : (help) Enzyme sequence for the IBS primer/gBlock : XbaI HindIII XhoI (help) Reference genome : none E.coli str. K-12 substr. MG1655 NC_000913 (help) Name of the target (14 characters max., letters and numbers only, no spaces, no underscore) : Authorization code : (case sensitive)