Enzyme sequence for the IBS primer/gBlock
The enzyme sequence (shown as XbaI) will be at the 5'- end of the retargeted PCR product or gBlockExample:
IBS1/2S CCCCTCTAGAAGAATTCCCATGCCAAACTGGTGCTCGAATGTGCGACATGAAGTCG
gBlock CCCCTCTAGAAGAATTCCCATGCCAAACTGGTGCTCGAATGTGCGA . . . etc.
Primer for gBlock extension (required):
EcI5-F CCCCTCTAGAAGAATTCCCATGCCAAA
