Enzyme sequence for the IBS primer/gBlock
The enzyme sequence (shown as HindIII) will be at the 5'- end of the retargeted PCR product or gBlockExample:
IBS1/2 AAAAAAGCTTATAATTATCCTTAGACCGCATGCTCGTGCGCCCAGATAGGGTG
gBlock TTCCCCTCTAGAAAAAAGCTTATAATTATCCTTAGA . . . etc.
primer for gBlock amplification (optional):
LtrB-F TTCCCCTCTAGAAAAAAGCTTATAATTATCCTTA
