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TargeTron
gBlock

Enzyme sequence for the IBS primer/gBlock

The enzyme sequence (shown as HindIII) will be at the 5'- end of the retargeted PCR product or gBlock

Example:

IBS1/2      AAAAAAGCTTATAATTATCCTTAGACCGCATGCTCGTGCGCCCAGATAGGGTG

gBlock      TTCCCCTCTAGAAAAAAGCTTATAATTATCCTTAGA . . . etc.

primer for gBlock amplification (optional):

LtrB-F      TTCCCCTCTAGAAAAAAGCTTATAATTATCCTTA